Key Features and Benefits

·Effective genomic DNA removal — without compromising cDNA synthesis efficiency

·Proprietary DNase buffer — maintaining RNA integrity during gDNA removal

·Convenient all-in-one, single-tube supermix — for reproducible and efficient cDNA synthesis

·Ready-to-use no-RT control supermix provided — for monitoring gDNA contamination

·Superior sensitivity and reproducibility — works with a broad linear dynamic range of input total RNA (1 µg–1 pg)

·Unbiased qPCR data — consistent efficiency, sensitivity, and reproducibility in qPCR results even after gDNA removal

gNA-free cDNA ready in 36 minutes

This kit allows you to effectively remove contaminating gDNA and reliably synthesize cDNA with minimal hands-on time in an easy-to-follow protocol, resulting in cDNA free of genomic DNA in 36 minutes.

Importance of Addressing gDNA Contamination in RT-qPCR Gene Expression

gDNA contamination in RNA samples can be amplified in RT-qPCR, biasing the results for the transcript of interest. This problem is especially acute for low expressing genes — even a few copies of gDNA contamination can shift Cq values dramatically.

Benefit of Using Reverse Transcriptase with RNase H+ in RT-qPCR Gene Expression

RNase H works to degrade RNA that is hybridized to DNA. During cDNA synthesis, the RNase H domain of the Moloney Murine Leukemia Virus (MMLV) reverse transcriptase degrades the template RNA as the cDNA is synthesized.

his activity ensures the one-to-one conversion of RNA into cDNA molecules, resulting in more accurate gene expression data. Reverse transcriptase in all iScript reverse transcription reagents exhibits RNase H activity.